Active Recombinant Mouse Chl1, His-tagged

• Cat.No.: Chl1-455M
• Product Overview: Recombinant Mouse Chl1 (Accession # BAC30699) Ala25-Gln1043 (Leu227-Gln242 del & Ala243Ser), fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 25-1043;227-242 a.a.
• Predicted N Terminal: Ala25
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic hippocampal neurons.Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.
• Molecular Mass: Recombinant Mouse Chl1, His-tagged has a calculated MW of 114 kDa. In SDS-PAGE migrates as 160-180 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Chl1 cell adhesion molecule with homology to L1CAM [ Mus musculus ]
• Official Symbol: Chl1
• Synonyms: CHL1; cell adhesion molecule with homology to L1CAM; neural cell adhesion molecule L1-like protein; chl1-like protein; close homolog of L1; CALL; LICAM2; AI465420; A530023M13Rik
• Gene ID: 12661
• mRNA Refseq: NM_007697
• Protein Refseq: NP_031723