Active Recombinant Mouse CST6 Protein, His-tagged

• Cat.No.: CST6-927M
• Product Overview: Recombinant extracellular domain of mouse CST6 (NP_082899.1) (Met 1-Ala 152) was expressed, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-152 a.a.
• Predicted N Terminal: Glu 29
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate ZFR-AMC. The IC50 is< 20 nM.
• Molecular Mass: The secreted recombinant mouse CST6 comprises 132 amino acids and has a calculated molecular mass of 15.2 kDa. As a result of glycosylation, the apparent molecular mass of rmCST6 is approximately 20 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Cst6 cystatin E/M [ Mus musculus ]
• Official Symbol: CST6
• Synonyms: CST6; cystatin E/M; cystatin-M; cystatin M; harlequin ichthyosis; harlequin ichthysosis; ichq; N28197; 1110017E11Rik
• Gene ID: 73720
• mRNA Refseq: NM_028623
• Protein Refseq: NP_082899