Active Recombinant Mouse FcRn Protein, His-tagged

• Cat.No.: FCGRT&B2M-02M
• Product Overview: Recombinant Mouse FcRn Protein (Ser22-Ser301) with a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Species: Mouse
• Source: NS0
• Tag: His
• Protein Length: Ser22-Ser301
• Description: Predicted to enable IgG binding activity and beta-2-microglobulin binding activity. Predicted to be involved in immune response. Predicted to be located in endosome and plasma membrane. Predicted to be integral component of membrane. Predicted to be active in external side of plasma membrane and extracellular space. Is expressed in several structures, including genitourinary system; intestine; liver; sensory organ; and skeleton. Orthologous to human FCGRT (Fc fragment of IgG receptor and transporter).
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Mouse FCRN is immobilized at 0.5 μg/mL (100 μL/well), the concentration of human IgG that produces 50% of the optimal binding response is found to be approximately 2-10 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 32 kDa (mFcRN); 12 kDa (m beta -2-microglobulin)
  SDS-PAGE: 41-46 kDa (mFcRN); 9-12 kDa (m beta -2-microglobulin)
• N-terminal Sequence Analysis: Ser22 (FcRN) & Ile21 ( beta -2-microglobulin)
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Fcgrt Fc fragment of IgG receptor and transporter [ Mus musculus (house mouse) ]
• Official Symbol: Fcgrt
• Synonyms: Fcgrt; Fc fragment of IgG receptor and transporter; FcRn; IgG receptor FcRn large subunit p51; Fc receptor, IgG, alpha chain transporter; igG Fc fragment receptor transporter alpha chain; neonatal Fc receptor
• Gene ID: 14132
• mRNA Refseq: NM_010189
• Protein Refseq: NP_034319
• UniProt ID: Q61559