Active Recombinant Mouse Frizzled Homolog 8 (Drosophila), Fc-tagged

• Cat.No.: Fzd8-593M
• Product Overview: RecombinantMouse Fzd8 protein was expressed in Murine myeloma cell line with the Fc partof Human IgG1 Pro100-Lys330 (C-terminus) and Mouse Frizzled-8 (Ala25-Pro173)&(Ala28-Pro173)(N-terminus).

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 25-173 a.a.
• Description: Fzd8 is one of atleast ten seventransmembrane (7TM) glycoprotein of the Frizzled family of Wntreceptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement,with low density lipoprotein receptor related proteins LRP-5 or LRP-6 actingas co-receptors stabilizes β-catenin and promotes gene transcription that isimportant in development and tissue maintenance.
• Form: Supplied as a 0.2 μmfiltered solution in PBS.
• N-terminal Sequence: Ala25 & Ala28
• Molecular Weight: 43 kDa
• Activity: Measured by itsability to interfere with the axis-inducing activity of Xwnt-8 mRNA in early Xenopusembryos. In a population of embryos, addition of 1 ng of mouse Frizzled8/ FcChimera protein per embryo resulted in 50-100% reduction in the number ofsecondary axes produced by 10-20 pg of Xwnt-8 mRNA. Also measured by itsability to bind biotinylated rmWnt-5a in a functional ELISA with an estimatedKD < 1 nM.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 59-60 kDa, reducingconditions
• Purity: >95%, by SDS-PAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 250μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to-70 °C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Fzd8 frizzled homolog 8(Drosophila) [ Mus musculus ]
• Official Symbol: Fzd8
• Synonyms: Fzd8;frizzled homolog 8 (Drosophila); Fz8; frizzled-8; Fz-8; mFz8
• Gene ID: 14370
• mRNA Refseq: NM_008058
• Protein Refseq: NP_032084
• UniProt ID: Q61091
• Chromosome Location: 18
• Pathway: Basal cellcarcinoma; Class B/2 (Secretin family receptors); GPCR ligand binding; HTLV-Iinfection; Melanogenesis; Pathways in cancer; Signal Transduction; Signalingby GPCR; Wnt Signaling Pathway; Wnt signaling network
• Function: G-proteincoupled receptor activity; PDZ domain binding; Wnt-activated receptoractivity; Wnt-protein binding