Active Recombinant Mouse Gla protein, His-tagged

• Cat.No.: Gla-7408M
• Product Overview: Recombinant Mouse Gla protein(Q8BGZ6)(Met1-Arg421), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg421
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze 4-methylumbelliferyl-α-D-galactopyranoside.The specific activity is > 400 pmoles/min/μg.
• Molecular Mass: The recombinant mouse Gla comprises 399 amino acids and has a predicted molecular mass of 45.6 kDa. The apparent molecular mass of the protein is approximately 46-52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gla galactosidase, alpha [ Mus musculus ]
• Official Symbol: Gla
• Synonyms: GLA; galactosidase, alpha; alpha-galactosidase A; melibiase; alpha-D-galactosidase A; alpha-D-galactoside galactohydrolase; Ags
• Gene ID: 11605
• mRNA Refseq: NM_013463
• Protein Refseq: NP_038491