Recombinant Full Length Human GLA Protein, C-Flag-tagged

• Cat.No.: GLA-1628HFL
• Product Overview: Recombinant Full Length Human GLA Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 45.3 kDa
• AA Sequence: MQLRNPELHLGCALALRFLALVSWD IPGARALDNGLARTPTMGWLHWERF MCNLDCQEEPDSCISEKLFM EMAELMVSEGWKDAGYEYLCIDDCW MAPQRDSEGRLQADPQRFPHGIRQL ANYVHSKGLKLGIYADVGNK TCAGFPGSFGYYDIDAQTFADWGVD LLKFDGCYCDSLENLADGYKHMSLA LNRTGRSIVYSCEWPLYMWP FQKPNYTEIRQYCNHWRNFADIDDS WKSIKSILDWTSFNQERIVDVAGPG GWNDPDMLVIGNFGLSWNQQ VTQMALWAIMAAPLFMSNDLRHISP QAKALLQDKDVIAINQDPLGKQGYQ LRQGDNFEVWERPLSGLAWA VAMINRQEIGGPRSYTIAVASLGKG VACNPACFITQLLPVKRKLGFYEWT SRLRSHINPTGTVLLQLENT
  MQMSLKDLLTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Galactose metabolism, Glycerolipid metabolism, Glycosphingolipid biosynthesis - globo series, Lysosome, Sphingolipid metabolism

Gene Information

• Gene Name: GLA galactosidase alpha [ Homo sapiens (human) ]
• Official Symbol: GLA
• Synonyms: GALA
• Gene ID: 2717
• mRNA Refseq: NM_000169.3
• Protein Refseq: NP_000160.1
• MIM: 300644
• UniProt ID: P06280