Active Recombinant Mouse Gpc5 Protein, His-tagged

• Cat.No.: Gpc5-859M
• Product Overview: Recombinant Mouse Gpc5 (Accession # NP_780709) Gly26-Gly549, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: Gly26-Gly549
• Predicted N Terminal: Gly26
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized rmGlypican 5 at 5 µg/mL (100 µL/well) can bind rhFGF-basic with a linear range of 0.16 - 10 ng/mL.
• Molecular Mass: Recombinant Mouse Gpc5, His-tagged has a calculated MW of 59.1 kDa. In SDS-PAGE migrates as 61-65 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Gpc5 glypican 5 [ Mus musculus ]
• Official Symbol: Gpc5
• Synonyms: GPC5; glypican 5; glypican-5; A230034F01Rik
• Gene ID: 103978
• mRNA Refseq: NM_175500
• Protein Refseq: NP_780709