Active Recombinant Mouse IFNAR2 protein, His-tagged

• Cat.No.: IFNAR2-0238M
• Product Overview: Recombinant Mouse IFNAR2 protein(Ser22-Ala242), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 22-242 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Mouse IFN-alpha / beta R2, His Tag on NTA Biosensor, can bind Human IFN-alpha 2b (K46R), Fc Tag with an affinity constant of 1.9 μM as determined in BLI assay (ForteBio Octet Red96e).Mouse IFN-alpha / beta R2, His Tag immobilized on CM5 Chip can bind Mouse IFN-alpha 1, His Tag with an affinity constant of 0.109 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser22-Ala242
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ifnar2 interferon (alpha and beta) receptor 2 [ Mus musculus ]
• Official Symbol: IFNAR2
• Synonyms: IFNAR2; interferon (alpha and beta) receptor 2; interferon alpha/beta receptor 2; IFN-R-2; interferon receptor 2c; IFN-alpha/beta receptor 2; type I interferon receptor 2; interferon-alpha/beta receptor 2; type I interferon alpha/beta receptor 2a; Ifnar-2; AI747302
• Gene ID: 15976
• mRNA Refseq: NM_001110498
• Protein Refseq: NP_001103968