Active Recombinant Mouse IGF1 protein, Fc-tagged

• Cat.No.: IGF1-0244M
• Product Overview: Recombinant Mouse IGF1 protein(Gly49-Ala118), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 49-118 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse IGF-I R, His Tag at 5 μg/mL (100 μL/well) can bind Mouse IGF-I Protein, Fc Tag with a linear range of 0.02-1.25 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminus.The protein has a calculated MW of 34.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gly49-Ala118
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Igf1 insulin-like growth factor 1 [ Mus musculus ]
• Official Symbol: IGF1
• Synonyms: IGF1; insulin-like growth factor 1; insulin-like growth factor I; somatomedin; Igf-1; Igf-I; C730016P09Rik
• Gene ID: 16000
• mRNA Refseq: NM_001111274
• Protein Refseq: NP_001104744