Active Recombinant Mouse Igf1 protein, His-GST-tagged

• Cat.No.: Igf1-1655M
• Product Overview: Active Recombinant Mouse Igf1 protein(Q8CAR0)(49~118 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: GST&His
• Protein Length: 49-118 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Insulin-like growth factor 1 (IGF1), also called somatomedin C is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF1, mecasermin, is used for the treatment of growth failure. Besides, Insulin Like Growth Factor Binding Protein 3 (IGFBP3) has been identified as an interactor of IGF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IGF1 and recombinant rat IGFBP3. Briefly, IGF1 were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100 μl were then transferred to IGFBP3-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IGF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of IGF1 and IGFBP3 was shown, and this effect was in a dose dependent manner, the EC50 was 1.06 ug/ml.
• Molecular Mass: 37.7kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Igf1 insulin-like growth factor 1 [ Mus musculus ]
• Official Symbol: Igf1
• Synonyms: IGF1; insulin-like growth factor 1; insulin-like growth factor I; somatomedin; Igf-1; Igf-I; C730016P09Rik
• Gene ID: 16000
• mRNA Refseq: NM_001111274
• Protein Refseq: NP_001104744