Active Recombinant Mouse Il17f, Met-tagged

• Cat.No.: Il17f-403M
• Product Overview: Recombinant Mouse Il17f (Accession # AAM77568) Arg21-Ala153, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 21-153 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its ability to bind mouse IL-17 RC in a functional ELISA with an estimated KD < 3 nM. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811.The ED50 for this effect is typically 20-120 ng/mL.
• Molecular Mass: Recombinant Mouse Il17f, Met-tagged has a calculated MW of 15 kDa (monomer). In SDS-PAGE migrates as 16 kDa, reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Il17f interleukin 17F [ Mus musculus ]
• Official Symbol: Il17f
• Synonyms: IL17F; interleukin 17F; interleukin-17F; C87042; IL-17F
• Gene ID: 257630
• mRNA Refseq: NM_145856
• Protein Refseq: NP_665855