Active Recombinant Mouse IL1A Protein

• Cat.No.: IL1A-149M
• Product Overview: Recombinant Mouse IL1A Protein without tag was expressed in E. coli.

Specification

• Description: Interleukin 1 alpha (IL-1α) is expressed by epithelial cells, activated macrophages, neutrophils, and endothelial cells to regulate immune responses. IL-1α signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1α and the independently regulated IL-1β protein have overlapping proinflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.
• Source: E. coli
• Species: Mouse
• Bio-activity: D10.G4.1 proliferation, ≤10 pg/mL
• Molecular Mass: Monomer, 18.1 kDa (157 aa)
• AA Sequence: MSAPYTYQSDLRYKLMKLVRQKFVM NDSLNQTIYQDVDKHYLSTTWLNDL QQEVKFDMYAYSSGGDDSKYPVTLK ISDSQLFVSAQGEDQPVLLKELPET PKLITGSETDLIFFWKSINSKNYFT SAAYPELFIATKEQSRVHLARGLPS MTDFQIS
• Endotoxin: ≤1 EUs/μg, Kinetic LAL
• Purity: ≥95%, Reducing and Non-Reducing SDS PAGE
• Stability: 12 months from date of receipt when stored at -20 to -80 centigrade as supplied.
  1 month when stored at 4 centigrade after reconstituting as directed.
  3 months when stored at -20 to -80 centigrade after reconstituting as directed.
• Storage: Storage Prior to Reconstitution: -20 centigrade
• Storage Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
• Reconstitution: Sterile water at 0.1 mg/mL
• Shipping: Room temperature
• Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws.

Gene Information

• Gene Name: Il1a interleukin 1 alpha [ Mus musculus (house mouse) ]
• Official Symbol: IL1A
• Synonyms: IL1A; interleukin 1 alpha; interleukin-1 alpha; IL-1 alpha; Il-1a
• Gene ID: 16175
• mRNA Refseq: NM_010554
• Protein Refseq: NP_034684
• UniProt ID: P01582

Reviews

04/08/2021

High solubility.

07/02/2020

Good for intracellular staining.

03/13/2019

Effective in apoptosis assays.

Q&As

What methods are employed to dissect the intricate cross-talk between IL-1α and other cytokines, shedding light on its role in immune modulation?

Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.

What experimental approaches allow researchers to dissect the paradoxical roles of IL-1α as both a pro-inflammatory and tissue repair mediator in distinct contexts?

IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.

How are cutting-edge techniques like single-cell RNA sequencing utilized to unravel the heterogeneity in IL-1α-responsive cell populations and their functional implications?

Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.

How is the intricate web of IL-1α's pro-inflammatory signaling pathways decoded using advanced experimental techniques?

IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.

How are state-of-the-art genetic editing tools such as CRISPR-Cas9 applied to unravel IL-1α's precise contributions to inflammatory diseases?

Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.

How do researchers manipulate in vitro co-culture systems to dissect the dynamic interplay between immune cells and stromal cells in IL-1α-mediated responses?

In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.

What experimental strategies enable the identification of specific cellular receptors and downstream effectors responsible for IL-1α's diverse physiological effects?

Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.

How are sophisticated imaging techniques like live-cell microscopy harnessed to capture the real-time dynamics of IL-1α release and its impact on neighboring cells?

Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.

How is the quantitative relationship between IL-1α levels and disease progression established through longitudinal studies and statistical modeling?

Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.

How do multi-omics analyses, including transcriptomics and proteomics, provide a holistic view of the network of molecules influenced by IL-1α signaling?

Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.