Active Recombinant Mouse Il1rapl1 protein(Met1-Thr357), hFc-tagged

• Cat.No.: Il1rapl1-6962M
• Product Overview: Recombinant Mouse IL1RAPL1 (P59823) extracellular domain (Met 1-Thr 357) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Protein length: Met1-Thr357
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind biotinylated human IL1α in functional ELISA.
• Molecular Mass: The secreted recombinant mouse IL1RAPL1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 574 amino acids and has a calculated molecular mass of 65 kDa. As a result of glycosylation, the apparent molecular mass of rmIL1RAPL1/Fc monomer is approximately 85-95 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il1rapl1 interleukin 1 receptor accessory protein-like 1 [ Mus musculus ]
• Official Symbol: Il1rapl1
• Synonyms: IL1RAPL1; interleukin 1 receptor accessory protein-like 1; interleukin-1 receptor accessory protein-like 1; IL1RAPL-1; IL-1RAPL-1; IL-1-RAPL-1; X-linked interleukin-1 receptor accessory protein-like 1; IL1R8; MRX34; OPHN4; IL1RAPL; TIGIRR-2; C030039N24; 6330532G10Rik
• Gene ID: 331461
• mRNA Refseq: NM_001160403
• Protein Refseq: NP_001153875

Reviews

07/08/2022

Effective in inflammation studies.

03/20/2020

High specificity.

07/30/2017

Suitable for cytokine assays.

Q&As

Can IL1RAPL1 mutations be linked to specific neurological disorders?

Linked to intellectual disabilities.

How does IL1RAPL1 expression vary in different brain regions?

Higher in the hippocampus and cortex.

How does IL1RAPL1 expression change during neural development?

Increases during early development, decreases in adulthood.

How does IL1RAPL1 interact with other members of the IL-1 receptor family?

Forms heterodimers with IL-1RI.

How does IL1RAPL1 interact with PSD-95 in neurons?

Binds directly to the PDZ domains of PSD-95.

What is the role of IL1RAPL1 in synaptic plasticity?

Involved in excitatory synapse formation.

Is IL1RAPL1 expression affected by neuroinflammatory conditions?

Upregulated in neuroinflammatory conditions.

Does IL1RAPL1 have any alternative splicing variants?

Yes, multiple isoforms exist.

What post-translational modifications occur on IL1RAPL1?

Subject to ubiquitination.

Does IL1RAPL1 have a role in dendritic spine morphology?

Affects spine density and shape.