Active Recombinant Mouse Il2 Protein, His-tagged

• Cat.No.: Il2-7239M
• Product Overview: Recombinant mouse IL2 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 21-169
• Description: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
• Form: Liquid
• Bio-activity: Measured in a cell proliferation assay using CTLL-2 mouse T lymphocyte. The ED50 for this effect is < 0.9 ng/mL.
• Molecular Mass: 17.3 kDa
• AA Sequence: MAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFSQSIISTSPQ
• Endotoxin: < 1.0 EU/μg of protein (determined by LAL method)
• Purity: > 90 % by SDS-PAGE
• Stability: Shelf life: one year from despatch.
• Storage: Store undiluted at 2-8 centigrade for one week or (in aliquots) at -20 to -80 centigrade for longer. Avoid repeated freezing and thawing.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: PBS, pH 7.4

Gene Information

• Gene Name: Il2 interleukin 2 [ Mus musculus (house mouse) ]
• Official Symbol: Il2
• Synonyms: Il2; interleukin 2; IL; Il-2; interleukin-2; T-cell growth factor; TCGF
• Gene ID: 16183
• mRNA Refseq: NM_008366
• Protein Refseq: NP_032392
• UniProt ID: P04351