Active Recombinant Mouse Il34 Protein (Asn21-Gln218), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il34-294M
• Product Overview: Recombinant Mouse Il34 Protein (Asn21-Gln218) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Asn21-Gln218
• Description: Interleukin 34 (IL-34) is a 23.98 kDa cytokine with 216 amino acid residues. IL-34 is a ligand of the colony-stimulating factor 1 receptor (CSF-1R) and is mainly secreted from the adrenal, frontal lobe, and cortex. IL-34 play a vital role in the proliferation, survival, differentiation, and migration of myeloid cells. These functions are mediated by IL-33 activating ERK1 /2, JAK, and PI3K/AKT signal pathways.
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is < 30 ng/mL. The specific activity of recombinant mouse IL-34 is > 3.3 × 10⁴ IU/mg.
• Molecular Mass: The protein has a calculated MW of 25.52 kDa.
  The protein migrates about 25 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il34 interleukin 34 [ Mus musculus (house mouse) ]
• Official Symbol: Il34
• Synonyms: IL34; interleukin 34; interleukin-34; IL-34; AI593503; 2010004A03Rik
• Gene ID: 76527
• mRNA Refseq: NM_001135100
• Protein Refseq: NP_001128572
• UniProt ID: Q8R1R4