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Recombinant Mouse IL34 cell lysate

Cat.No. : IL34-2783MCL
Product Overview : Mouse IL-34 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Il34 interleukin 34 [ Mus musculus ]
Official Symbol : IL34
Synonyms : IL34; interleukin 34; interleukin-34; IL-34; AI593503; 2010004A03Rik;
Gene ID : 76527
mRNA Refseq : NM_001135100
Protein Refseq : NP_001128572
Function : cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; macrophage colony-stimulating factor receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the effect of IL34 on tumor prognosis? 09/19/2022

There are no clear studies showing the effect of IL34 on tumor prognosis.

What are the advantages of IL34 over other treatments? 05/14/2022

Can fight disease by modulating the immune system and may have better efficacy and fewer side effects than other treatments.

What is the mechanism of action of IL34 in the treatment of tumor? 07/09/2020

May inhibit tumor growth and metastasis by enhancing immune cells' ability to attack tumor cells.

How is the clinical trial of IL34 progressing? 04/02/2020

No large-scale clinical trials have been reported, but some early studies have shown a potential therapeutic effect.

How does IL34 affect the immune system? 03/26/2020

Regulate the function of macrophages and dendritic cells and affect the immune response of the immune system.

How to evaluate the efficacy of IL34? 01/31/2019

The evaluation of the efficacy of IL34 usually requires a comprehensive evaluation of clinical symptoms, biochemical indicators, imaging and other indicators.

Customer Reviews (3)

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Reviews
05/10/2021

    IL34 has shown good biocompatibility and safety in related experiments.

    01/19/2020

      When using IL34 for experiments, the experimental conditions and results are highly repeatable.

      10/24/2019

        Quality is stable and reliable, with no significant differences between batches.

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