Active Recombinant Mouse Il4
Cat.No. : | Il4-297M |
Product Overview : | Recombinant Mouse Il4 was expressed in CHO Cells. |
Availability | February 15, 2025 |
Unit | |
Price | |
Qty |
- Specification
- Gene Information
- Related Products
- Citation
- Download
Description : | Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet. |
Source : | CHO Cells |
Species : | Mouse |
Form : | Lyophilized after extensive dialysis against PBS. |
Bio-activity : | ED50< 2 ng/ml, measured in a cell proliferation assay using murine ht-2 cells, corresponding to a specific activity of>5 x 105 units/mg. |
Molecular Mass : | 15 kDa, observed by reducing SDS-PAGE. |
AA Sequence : | His23-Ser140. |
Endotoxin : | < 0.2 eu/μg, determined by lal> |
Purity : | >95% as analyzed by SDS-PAGE and HPLC. |
Storage : | Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-4 should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Reconstitution : | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Tag : | Non |
Gene Name : | Il4 interleukin 4 [ Mus musculus ] |
Official Symbol : | Il4 |
Synonyms : | IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1; |
Gene ID : | 16189 |
mRNA Refseq : | NM_021283 |
Protein Refseq : | NP_067258 |
UniProt ID : | P07750 |
Pathway : | Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; |
Function : | cytokine activity; cytokine receptor binding; growth factor activity; interleukin-4 receptor binding; |
Products Types
◆ Lysates | ||
IL4-001MCL | Recombinant Mouse IL4 cell lysate | +Inquiry |
Related Gene
Parkinsonian neurotoxicants impair the anti-inflammatory response induced by IL4 in glial cells: involvement of the CD200-CD200R1 ligand-receptor pair
Journal: Scientific Reports PubMed ID: 32606391 Data: 2020/6/30
Authors: Neus Rabaneda-Lombarte, Lucas Blasco-Agell, Carme Solà
Article Snippet:Cells were treated with 50 ng/mL IL4 for 24 h in the absence or presence of MPP+ or rotenone.Cells were treated with 50 ng/mL IL4 for 24 h in the absence or presence of MPP+ or rotenone.. Recombinant mouse IL4 expressed in CHO cells was used (Creative BioMart, Shirley, NY, USA).. A stock solution of 50 mg/mL IL4 in a mixture of milli-Q H 2 O:culture medium (1:1) was prepared and stored at – 20 °C.A stock solution of 50 mg/mL IL4 in a mixture of milli-Q H 2 O:culture medium (1:1) was prepared and stored at – 20 °C.

Effect of MPP+ treatment on the mRNA expression of anti-inflammatory markers. IL10, TGFβ, IL1ra, Arg1, MR, Fizz1 and Ym1 mRNA expression in primary ( a ) mixed glial cultures and ( b ) microglial cultures treated for 24 h with 10 or 25 μM MPP+, in the absence and presence of

Effect of rotenone treatment on the mRNA expression of anti-inflammatory markers. IL10, TGFβ, IL1ra, Arg1, MR, Fizz1 and Ym1 mRNA expression in primary ( a ) mixed glial cultures and ( b ) microglial cultures treated for 24 h with 40 or 100 nM rotenone (Rot), in the absence and presence of

ARG1 and MR protein levels in primary glial cell cultures treated with MPP+ or rotenone. ARG1 and MR levels were determined in the total protein extracts from ( a , c ) mixed glial cultures and ( b , d ) microglial cultures treated with 10 or 25 μM MPP+ or 40 or 100 nM rotenone (Rot) for 24 h, in the absence or presence of
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Customer Reviews (3)
Write a reviewBy testing immunogenicity in animal models, detect whether an immune response is induced.
The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.
This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.
Ask a Question for All Il4 Products
Required fields are marked with *
My Review for All Il4 Products
Required fields are marked with *
Inquiry Basket