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Active Recombinant Mouse Il4

Cat.No. : Il4-297M
Product Overview : Recombinant Mouse Il4 was expressed in CHO Cells.
Availability February 15, 2025
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Description : Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet.
Source : CHO Cells
Species : Mouse
Form : Lyophilized after extensive dialysis against PBS.
Bio-activity : ED50< 2 ng/ml, measured in a cell proliferation assay using murine ht-2 cells, corresponding to a specific activity of>5 x 105 units/mg.
Molecular Mass : 15 kDa, observed by reducing SDS-PAGE.
AA Sequence : His23-Ser140.
Endotoxin : < 0.2 eu/μg, determined by lal>
Purity : >95% as analyzed by SDS-PAGE and HPLC.
Storage : Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-4 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Reconstitution : Reconstituted in ddH2O or PBS at 100 μg/ml.
Tag : Non
Gene Name : Il4 interleukin 4 [ Mus musculus ]
Official Symbol : Il4
Synonyms : IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1;
Gene ID : 16189
mRNA Refseq : NM_021283
Protein Refseq : NP_067258
UniProt ID : P07750
Pathway : Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem;
Function : cytokine activity; cytokine receptor binding; growth factor activity; interleukin-4 receptor binding;

Parkinsonian neurotoxicants impair the anti-inflammatory response induced by IL4 in glial cells: involvement of the CD200-CD200R1 ligand-receptor pair

Journal: Scientific Reports    PubMed ID: 32606391    Data: 2020/6/30

Authors: Neus Rabaneda-Lombarte, Lucas Blasco-Agell, Carme Solà

Article Snippet:Cells were treated with 50 ng/mL IL4 for 24 h in the absence or presence of MPP+ or rotenone.Cells were treated with 50 ng/mL IL4 for 24 h in the absence or presence of MPP+ or rotenone.. Recombinant mouse IL4 expressed in CHO cells was used (Creative BioMart, Shirley, NY, USA).. A stock solution of 50 mg/mL IL4 in a mixture of milli-Q H 2 O:culture medium (1:1) was prepared and stored at – 20 °C.A stock solution of 50 mg/mL IL4 in a mixture of milli-Q H 2 O:culture medium (1:1) was prepared and stored at – 20 °C.

Effect of MPP+ treatment on the mRNA expression of anti-inflammatory markers. IL10, TGFβ, IL1ra, Arg1, MR, Fizz1 and Ym1 mRNA expression in primary ( a ) mixed glial cultures and ( b ) microglial cultures treated for 24 h with 10 or 25 μM MPP+, in the absence and presence of IL4 (50 ng/mL). Genes encoding Rn18s and β-actin were used as the reference genes. Bars correspond to the means + SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); # p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test. & p < 0.05, && p < 0.01 and &&& p < 0.001 vs C, one-way ANOVA and Newman-Keuls post-hoc test considering only the IL4-free groups, which was performed to detect whether the high values observed in the IL4 group hindered the detection of a statistical significance for the effects of MPP+ alone.

Effect of MPP+ treatment on the mRNA expression of anti-inflammatory markers. IL10, TGFβ, IL1ra, Arg1, MR, Fizz1 and Ym1 mRNA expression in primary ( a ) mixed glial cultures and ( b ) microglial cultures treated for 24 h with 10 or 25 μM MPP+, in the absence and presence of IL4 (50 ng/mL). Genes encoding Rn18s and β-actin were used as the reference genes. Bars correspond to the means + SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); # p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test. & p < 0.05, && p < 0.01 and &&& p < 0.001 vs C, one-way ANOVA and Newman-Keuls post-hoc test considering only the IL4-free groups, which was performed to detect whether the high values observed in the IL4 group hindered the detection of a statistical significance for the effects of MPP+ alone.

Effect of rotenone treatment on the mRNA expression of anti-inflammatory markers. IL10, TGFβ, IL1ra, Arg1, MR, Fizz1 and Ym1 mRNA expression in primary ( a ) mixed glial cultures and ( b ) microglial cultures treated for 24 h with 40 or 100 nM rotenone (Rot), in the absence and presence of IL4 (50 ng/mL). Genes encoding Rn18s and β-actin were used as the reference genes. Bars correspond to the means+ SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); # p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test. &&& p < 0.001 vs C, one-way ANOVA and Newman-Keuls post-hoc test considering only the IL4-free groups, which was performed to detect whether the high values observed in the IL4 group hindered the detection of a statistical significance of the effects of Rot alone.

Effect of rotenone treatment on the mRNA expression of anti-inflammatory markers. IL10, TGFβ, IL1ra, Arg1, MR, Fizz1 and Ym1 mRNA expression in primary ( a ) mixed glial cultures and ( b ) microglial cultures treated for 24 h with 40 or 100 nM rotenone (Rot), in the absence and presence of IL4 (50 ng/mL). Genes encoding Rn18s and β-actin were used as the reference genes. Bars correspond to the means+ SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); # p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test. &&& p < 0.001 vs C, one-way ANOVA and Newman-Keuls post-hoc test considering only the IL4-free groups, which was performed to detect whether the high values observed in the IL4 group hindered the detection of a statistical significance of the effects of Rot alone.

ARG1 and MR protein levels in primary glial cell cultures treated with MPP+ or rotenone. ARG1 and MR levels were determined in the total protein extracts from ( a , c ) mixed glial cultures and ( b , d ) microglial cultures treated with 10 or 25 μM MPP+ or 40 or 100 nM rotenone (Rot) for 24 h, in the absence or presence of IL4 (50 ng/mL). Images of representative western blots are presented. Bars correspond to the means+ SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); #p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test.

ARG1 and MR protein levels in primary glial cell cultures treated with MPP+ or rotenone. ARG1 and MR levels were determined in the total protein extracts from ( a , c ) mixed glial cultures and ( b , d ) microglial cultures treated with 10 or 25 μM MPP+ or 40 or 100 nM rotenone (Rot) for 24 h, in the absence or presence of IL4 (50 ng/mL). Images of representative western blots are presented. Bars correspond to the means+ SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); #p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test.

Publication :
LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy (2019)
Alterations in the development of a M2 phenotype in glial cells: rotenone in vitro model of Parkinson's disease (2017)
Altered expression of the immunoregulatory ligand-receptor pair CD200-CD200R1 in the brain of Parkinson’s disease patients (2022)
Parkinsonian neurotoxicants impair the anti-inflammatory response induced by IL4 in glial cells: involvement of the CD200-CD200R1 ligand-receptor pair (2020)

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10/17/2021

    By testing immunogenicity in animal models, detect whether an immune response is induced.

    11/09/2020

      The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

      05/06/2020

        This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.

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