Active Recombinant Mouse Il6 Protein (Phe25-Thr211), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il6-61M
• Product Overview: Recombinant Mouse Il6 Protein (Phe25-Thr211) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin-6 (IL-6) is a pleiotropic, 22-28 kDa cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.
• Source: E. coli
• Species: Mouse
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in 7TD1 cells. The ED50 for this effect is < 0.01 ng/mL. The specific activity of recombinant mouse IL-6 is approximately >1 × 10⁸ IU/mg.
• Molecular Mass: The protein has a calculated MW of 22.7 kDa.
  The protein migrates as 24 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Phe25-Thr211
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il6 interleukin 6 [ Mus musculus (house mouse) ]
• Official Symbol: Il6
• Synonyms: IL6; interleukin 6; interleukin-6; interleukin HP-1; B-cell hybridoma growth factor; Il-6
• Gene ID: 16193
• mRNA Refseq: NM_031168
• Protein Refseq: NP_112445
• UniProt ID: P08505