Active Recombinant Mouse Interleukin 12b
Cat.No. : | Il12b-643M |
Product Overview : | RecombinantMouse Il12b protein was expressed in Spodopterafrugiperda. Met23-Ser335. |
- Specification
- Gene Information
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Cat. No. : | Il12b-643M |
Description : | Il12b is apleiotropic cytokine originally identified in the medium of activated human Blymphoblastoid cell lines. Il12b has multiple effectson T cells and NK cells and is a key mediator in the development of Th1cells. Il12b is aheterodimeric cytokine containing two disulfidelinked subunits, p35 and p40. |
Source : | Spodopterafrugiperda,Sf 21 (baculovirus)-derived. |
Form : | Lyophilizedfrom a 0.2 um filtered solution in PBS with BSA as a carrier protein. |
N-terminalSequence : | Met23 |
MolecularWeight : | 35.8 kDa (monomer) |
Activity : | Measuredby its ability to inhibit the Il12b dependentproliferation of mouse splenocytes activated with PHA and IL-2. The ED50 forthis effect is typically 1-3 ng/mL in the presence of 0.3 ng/mL of mouse Il12b. |
Endotoxin Level : | <1.0EU per 1μg of the protein by the LAL method. |
Purity : | >97%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : | Reconstituteat 10μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin. |
Storage : | Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution. |
Gene Name : | Il12b interleukin 12b [ Musmusculus ] |
Official Symbol : | Il12b |
Synonyms : | Il12b; interleukin12b; p40;Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40; IL-12subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDasubunit |
Gene ID : | 16160 |
mRNA Refseq : | NM_008352 |
Protein Refseq : | NP_032378 |
UniProt ID : | P43432 |
Chromosome Location : | 11 |
Pathway : | Africantrypanosomiasis; Allograft rejection; Amoebiasis; Cytokine-cytokine receptorinteraction; Herpes simplex infection; Influenza A; Jak-STAT signalingpathway; Legionellosis; Leishmaniasis; Measles; Pertussis; RIG-I-likereceptor signaling pathway; Toll-like receptor signaling pathway; Toxoplasmosis;Type I diabetes mellitus |
Function : | contributes_tocytokine activity; cytokine receptor activity; contributes_to cytokinereceptor binding; identical protein binding; interleukin-12 alpha subunitbinding; protein binding; protein heterodimerization activity |
Products Types
◆ Recombinant Protein | ||
Il12b-48M | Active Recombinant Mouse Il12b Protein (Met23-Ser335), C-His tagged, Animal-free, Carrier-free | +Inquiry |
IL12B-181I | Active Recombinant Human IL12B Protein | +Inquiry |
IL12B-5F | Recombinant Feline IL12B Protein, His (Fc)-Avi-tagged | +Inquiry |
IL12B-576S | Recombinant Sheep IL12B Protein (23-327 aa), His-tagged | +Inquiry |
Il12b-1237M | Recombinant Mouse Il12b Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
IL12B-1197RCL | Recombinant Rat IL12B cell lysate | +Inquiry |
IL12B-2731HCL | Recombinant Human IL12B cell lysate | +Inquiry |
IL12B-1101MCL | Recombinant Mouse IL12B cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
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