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Active Recombinant Mouse Interleukin 12b

Cat.No. : Il12b-643M
Product Overview : RecombinantMouse Il12b protein was expressed in Spodopterafrugiperda. Met23-Ser335.
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  • Gene Information
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Cat. No. : Il12b-643M
Description : Il12b is apleiotropic cytokine originally identified in the medium of activated human Blymphoblastoid cell lines. Il12b has multiple effectson T cells and NK cells and is a key mediator in the development of Th1cells. Il12b is aheterodimeric cytokine containing two disulfidelinked subunits, p35 and p40.
Source : Spodopterafrugiperda,Sf 21 (baculovirus)-derived.
Form : Lyophilizedfrom a 0.2 um filtered solution in PBS with BSA as a carrier protein.
N-terminalSequence : Met23
MolecularWeight : 35.8 kDa (monomer)
Activity : Measuredby its ability to inhibit the Il12b dependentproliferation of mouse splenocytes activated with PHA and IL-2. The ED50 forthis effect is typically 1-3 ng/mL in the presence of 0.3 ng/mL of mouse Il12b.
Endotoxin Level : <1.0EU per 1μg of the protein by the LAL method.
Purity : >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstituteat 10μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
Storage : Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
Gene Name : Il12b interleukin 12b [ Musmusculus ]
Official Symbol : Il12b
Synonyms : Il12b; interleukin12b; p40;Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40; IL-12subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDasubunit
Gene ID : 16160
mRNA Refseq : NM_008352
Protein Refseq : NP_032378
UniProt ID : P43432
Chromosome Location : 11
Pathway : Africantrypanosomiasis; Allograft rejection; Amoebiasis; Cytokine-cytokine receptorinteraction; Herpes simplex infection; Influenza A; Jak-STAT signalingpathway; Legionellosis; Leishmaniasis; Measles; Pertussis; RIG-I-likereceptor signaling pathway; Toll-like receptor signaling pathway; Toxoplasmosis;Type I diabetes mellitus
Function : contributes_tocytokine activity; cytokine receptor activity; contributes_to cytokinereceptor binding; identical protein binding; interleukin-12 alpha subunitbinding; protein binding; protein heterodimerization activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation? 05/12/2021

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity? 05/02/2021

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells? 10/30/2020

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation? 05/25/2020

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies? 04/30/2020

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners? 04/12/2018

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function? 12/03/2017

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets? 10/31/2017

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation? 09/29/2017

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context? 08/22/2017

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.

Customer Reviews (3)

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Reviews
04/08/2020

    Good for cytokine assays.

    01/06/2020

      Effective in Th2 cell differentiation.

      01/12/2019

        High yield in expression.

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