Active Recombinant Mouse Interleukin 27

• Cat.No.: Il27-506M
• Product Overview: Recombinant mouse p28 is a single-chain 207 amino acid peptide with a mass of approximately 23,697 Daltons.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Recombinant Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.
• Form: Lyophilized at a concentration of 1 mg/ml in 10mM NaP, 7.5
• Purity: Purity ~ 97% as determined by: Reducing and non-reducing SDS-PAGE.
• Bio-activity: Mouse p28 biological activity was measured via dose-dependent inhibition of TGF-beta & IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50ng/ml of mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg"s/ml, which can be further diluted into other aqueous solutions.
• Purity ~ 97% as determined by: Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLSDSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEEEEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus ]
• Official Symbol: Il27
• Synonyms: Il27; interleukin 27; p28; Il30; IL-27; IL-27p28; interleukin 30; IL-27 p28 subunit
• Gene ID: 246779
• mRNA Refseq: NM_145636
• Protein Refseq: NP_663611
• UniProt ID: Q8K3I6
• Chromosome Location: 7 F3
• Function: cytokine activity; interleukin-27 receptor binding; protein binding; receptor binding