Active Recombinant Mouse Masp1 Protein, His-tagged

• Cat.No.: Masp1-11122M
• Product Overview: Mouse MASP3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 455 - Arg 733 (Accession # BAB69688.1). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Ile 455

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 455-733 aa
• Description: MASP3 is a member of the MASP family. The MASP family proteins were first discovered as complexes with mannose-binding lectin (MBL) and therefore named MBL-associated serine proteases, but later, they were found to interact with ficolins, and later still, collectin-10 and collectin-11. As well as proteolytic enzymes (MASP-1, MASP-2, MASP-3), the group includes non-enzymatic factors (MAp19, MAp44). In this review, the association-specific factors of the lectin pathway with haematologic malignancies and related infections are discussed.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: The purity of Mouse MASP3 Protein, His Tag is more than 95% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
• Molecular Mass: The protein has a calculated MW of 32.6 kDa. The protein migrates as 40-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Masp1 MBL associated serine protease 1 [ Mus musculus (house mouse) ]
• Official Symbol: MASP3
• Synonyms: Masp1; MBL associated serine protease 1; CCPII; Crarf; Masp1/3; mannan-binding lectin serine protease 1; MASP-1; complement factor MASP-3; complement-activating component of Ra-reactive factor; mannan-binding lectin serine peptidase 1; mannose-binding lectin-associated serine protease 1; mannose-binding protein-associated serine protease; ra-reactive factor serine protease p100; raRF; serine protease 5; EC 3.4.21.-
• Gene ID: 17174
• mRNA Refseq: NM_008555
• Protein Refseq: NP_032581
• UniProt ID: P98064