Recombinant Human MASP1 Protein (20-699 aa), His-tagged

• Cat.No.: MASP1-1451H
• Product Overview: Recombinant Human MASP1 Protein (20-699 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Immunology. Protein Description: Full Length of Mature Protein.

Specification

• Description: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 79.0 kDa
• Protein length: 20-699 aa
• AA Sequence: HTVELNNMFGQIQSPGYPDSYPSDS EVTWNITVPDGFRIKLYFMHFNLES SYLCEYDYVKVETEDQVLATFCGRE TTDTEQTPGQEVVLSPGSFMSITFR SDFSNEERFTGFDAHYMAVDVDECK EREDEELSCDHYCHNYIGGYYCSCR FGYILHTDNRTCRVECSDNLFTQRT GVITSPDFPNPYPKSSECLYTIELE EGFMVNLQFEDIFDIEDHPEVPCPY DYIKIKVGPKVLGPFCGEKAPEPIS TQSHSVLILFHSDNSGENRGWRLSY RAAGNECPELQPPVHGKIEPSQAKY FFKDQVLVSCDTGYKVLKDNVEMDT FQIECLKDGTWSNKIPTCKIVDCRA PGELEHGLITFSTRNNLTTYKSEIK YSCQEPYYKMLNNNTGIYTCSAQGV WMNKVLGRSLPTCLPVCGLPKFSRK LMARIFNGRPAQKGTTPWIAMLSHL NGQPFCGGSLLGSSWIVTAAHCLHQ SLDPEDPTLRDSDLLSPSDFKIILG KHWRLRSDENEQHLGVKHTTLHPQY DPNTFENDVALVELLESPVLNAFVM PICLPEGPQQEGAMVIVSGWGKQFL QRFPETLMEIEIPIVDHSTCQKAYA PLKKKVTRDMICAGEKEGGKDACAG DSGGPMVTLNRERGQWYLVGTVSWG DDCGKKDRYGVYSYIHHNKDWIQRV TGVRN
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: MASP1 mannan binding lectin serine peptidase 1 [ Homo sapiens (human) ]
• Official Symbol: MASP1
• Synonyms: 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
• Gene ID: 5648
• mRNA Refseq: NM_001031849
• Protein Refseq: NP_001027019
• UniProt ID: P48740