Recombinant Full Length Human MASP1 Protein, C-Flag-tagged

• Cat.No.: MASP1-668HFL
• Product Overview: Recombinant Full Length Human MASP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 49 kDa
• AA Sequence: MRWLLLYYALCFSLSKASAHTVELN NMFGQIQSPGYPDSYPSDSEVTWNI TVPDGFRIKLYFMHFNLESS YLCEYDYVKVETEDQVLATFCGRET TDTEQTPGQEVVLSPGSFMSITFRS DFSNEERFTGFDAHYMAVDV DECKEREDEELSCDHYCHNYIGGYY CSCRFGYILHTDNRTCRVECSDNLF TQRTGVITSPDFPNPYPKSS ECLYTIELEEGFMVNLQFEDIFDIE DHPEVPCPYDYIKIKVGPKVLGPFC GEKAPEPISTQSHSVLILFH SDNSGENRGWRLSYRAAGNECPELQ PPVHGKIEPSQAKYFFKDQVLVSCD TGYKVLKDNVEMDTFQIECL KDGTWSNKIPTCKIVDCRAPGELEH GLITFSTRNNLTTYKSEIKYSCQEP YYKMLNNNTGIYTCSAQGVW MNKVLGRSLPTCLPVCGLPKFSRKL MARIFNGRPAQKGTTPWIAMLSHLN GQPFCGGSLLGSSWIVTAAH CLHQSLDPEDPTLRDSDLLSPSDFK IILGKHWRLRSDENEQHLGVKHTTL HPQYDPNTFENDVALVELLE SPVLNAFVMPICLPEGPQQEGAMVI VSGWGKQFLQRFPETLMEIEIPIVD HSTCQKAYAPLKKKVTRDMI CAGEKEGGKDACAGDSGGPMVTLNR ERGQWYLVGTVSWGDDCGKKDRYGV YSYIHHNKDWIQRVTGVRNTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Complement and coagulation cascades

Gene Information

• Gene Name: MASP1 MBL associated serine protease 1 [ Homo sapiens (human) ]
• Official Symbol: MASP1
• Synonyms: 3MC1; MAP1; MASP; RaRF; CRARF; MAP-1; MASP3; MAp44; PRSS5; CRARF1; MASP-3
• Gene ID: 5648
• mRNA Refseq: NM_001879.6
• Protein Refseq: NP_001870.3
• MIM: 600521
• UniProt ID: P48740