Active Recombinant Mouse Mrc1

• Cat.No.: Mrc1-12M
• Product Overview: Recombinant Mouse Mrc1(Leu19-Ala1388), fused with a C-terminal 6-His tag, was expressed in Mouse NSO Cells.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Non
• Protein Length: 19-1388 a.a.
• Description: The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity.
• Predicted N Terminal: Leu19
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to bind with Mannan in a functional ELISA.
• Molecular Mass: The recombinant Mouse Mrc1 predicts a molecular mass of 156 kDa (monomer). It migrates as an approximately 180 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 0.10 eu per 1 μg of the protein by the lal
• Purity: >90%, by SDS-PAGE
• Storage: Store it under sterile conditions at -20℃ to -70℃. It is recommended that the protein be aliquoted for optimal storage.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: MRC1 mannose receptor, C type 1 [ Homo sapiens ]
• Official Symbol: Mrc1
• Synonyms: MRC1; mannose receptor, C type 1; mannose receptor, C type 1 like 1 , MRC1L1; macrophage mannose receptor 1; bA541I19.1; CD206; CLEC13D; CLEC13DL; mannose receptor, C type 1-like 1; C-type lectin domain family 13 member D; macrophage mannose receptor 1-like protein 1; MMR; MRC1L1; MGC181940
• Gene ID: 4360
• mRNA Refseq: NM_002438
• Protein Refseq: NP_002429
• MIM: 153618
• UniProt ID: P22897
• Chromosome Location: 10p13
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cross-presentation of soluble exogenous antigens (endosomes), organism-specific biosystem; Immune System, organism-specific biosystem; Phagosome, organism-specific biosystem
• Function: binding; mannose binding; receptor activity; sugar binding

Citation

Synthesis, Formulation and Characterization of Immunotherapeutic Glycosylated Dendrimer/cGAMP Complexes for CD206 Targeted Delivery to M2 Macrophages in Cold Tumors

Journal: Pharmaceutics    PubMed ID: 36145631    Data: 2022/9/6

Authors: Marija Petrovic, Alexandre Porcello, Ilaria Clemente

Article Snippet:All further dilutions were also prepared in 1 × 20 mM HEPES (pH 7.4) supplemented with 0.1% pluronic F127.All further dilutions were also prepared in 1 × 20 mM HEPES (pH 7.4) supplemented with 0.1% pluronic F127.. Dye: 90 μL of 100 nM and 90 μL of 16 nM Recombinant Mouse Mrc1 (CD206) protein (Leu225 Ser492, His and T7-tagged #Mrc1-7099M Creative BioMart ? ), where the fluorescent signals of CD206 correspond to the typical detection limit of the Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany).. The mixture was incubated for 30 min at room temperature.The mixture was incubated for 30 min at room temperature.

NCs upregulate mRNA expression of pro-inflammatory (M1) genes and reduce the expression of the M2-associated gene Mrc1 in M2 polarized BMDMs. Relative mRNA expression of M2-like macrophage markers ( Mrc1 ( a ) and Arg1 ( b )) and of classically activated (M1-like) macrophage markers ( IL-1b ( c ), STAT1 ( d ) and NOS2 ( e )) in M2 BMDMs treated with corresponding PAMAM NCs (PG3 1/1, PG3 2/1, PG4 1/1 or PG4 2/1), cGAMP (25 μg/condition) or with media. * p < 0.05; ** p < 0.01; **** p < 0.0001.