| Species : |
Mouse |
| Source : |
Mouse myeloma cell line |
| Tag : |
His |
| Protein Length : |
22-603 aa |
| Description : |
Predicted to be an extracellular matrix structural constituent. Involved in chemorepulsion of axon and regulation of synapse assembly. Acts upstream of or within several processes, including generation of neurons; inner ear morphogenesis; and regulation of axon extension. Located in basement membrane and cytoplasm. Is active in glutamatergic synapse. Is expressed in several structures, including alimentary system; central nervous system; heart; lung; and sensory organ. Human ortholog(s) of this gene implicated in mirror movements 4. Orthologous to human NTN1 (netrin 1). |
| Form : |
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with BSA as a carrier protein. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized recombinant rat UNC5H2 Fc Chimera at 5 μg/mL (100 μL/well) can bind Recombinant Mouse Netrin-1 with a linear range of 3-200 ng/mL. |
| Molecular Mass : |
68.2 kDa |
| Endotoxin : |
< 0.10 EU/μg of the protein by the LAL method. |
| Purity : |
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining. |
| Applications : |
Binding Activity |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Shipping : |
The product is shipped at ambient temperature. |
| Reconstitution : |
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| N-terminal Sequence Analysis : |
Val22 |
