Active Recombinant Mouse Pro-Platelet Basic Protein

• Cat.No.: Ppbp-392M
• Product Overview: Recombinantmouse Ppbp protein was expressed in E. coli. Ile48-Ile109.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 48-109 a.a.
• Description: Ppbp is a proteinthat is released in large amounts from platelets following their activation.It stimulates various processes including mitogenesis, synthesis ofextracellular matrix, glucose metabolism and synthesis of plasminogenactivator.
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminal Sequence: IIe48
• Molecular Weight: 6.8 kDa
• Activity: Measured by itsability to chemoattract human CXCR2 transfected BaF3 mouse pro-B cells. TheED50 for this effect is typically 1-5 ng/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 10μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Ppbp pro-plateletbasic protein [ Mus musculus ]
• Official Symbol: Ppbp
• Synonyms: Ppbp; pro-plateletbasic protein; TGB; LDGF; MDGF; TGB1; CTAP3; Cxcl7; NAP-2; Scyb7;b-TG1; LA-PF4; THBGB1; CTAPIII; beta-TG; AI854500; NAP-2-L1; 2400003M24Rik; plateletbasic protein; chemokine (C-X-C) ligand 7; chemokine (C-X-C) ligand 7,isoform CRA_b; chemokine subfamily B Cys-X-Cys; Thymus chemokine 1;Uncharacterized protein; mCG_1702
• Gene ID: 57349
• mRNA Refseq: NM_023785
• Protein Refseq: NP_076274
• UniProt ID: Q9EQI5
• Chromosome Location: 5
• Pathway: Chemokine receptorsbind chemokines; Chemokine signaling pathway; Class A/1 (Rhodopsin-likereceptors); Cytokine-cytokine receptor interaction; G alpha (i) signallingevents; GPCR downstream signaling; GPCR ligand binding; Hemostasis; Peptideligand-binding receptors; Platelet activation, signaling and aggregation;Platelet degranulation; Response to elevated platelet cytosolic Ca2+;Signal Transduction; Signaling by GPCR
• Function: Molecular function

Citation

Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity

Journal: Cell reports    PubMed ID: 35263574    Data: 2022/4/25

Authors: Jocelyn G. Labombarde, Meenu R. Pillai, Maureen A. McGargill

Article Snippet:Mice received rapamycin or PBS for 14 days, and then were rested for two weeks without additional injections to be sure the rapamycin was not present at the time of EAE induction.Mice received rapamycin or PBS for 14 days, and then were rested for two weeks without additional injections to be sure the rapamycin was not present at the time of EAE induction.. EAE was induced by injecting mice subcutaneously (s.c.) with an emulsion of 100 μg of mouse native myelin basic protein (Creative BioMart) with 400 μg of CFA (Chondrex).. Mice also received 200 ng of pertussis toxin (List Biologicals) i.p. at the same time as induction, and two days later.Mice also received 200 ng of pertussis toxin (List Biologicals) i.p. at the same time as induction, and two days later.

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