Active Recombinant Mouse Prss3 Protein, Met & His-tagged

• Cat.No.: Prss3-663M
• Product Overview: Recombinant Mouse Prss3 (Accession # NP_035775) Ile24-Asn246, fused with an N-terminal Met, 6-His tag and GGSGSGGGSSR linker, was produced in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Ile24-Asn246
• Predicted N Terminal: Ile24
• Form: Supplied as a 0.2 µm filtered solution in Sodium Acetate, NaCl, CaCl2, BME, DTT and Glycerol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The specific activity is >30,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Prss3, Met & His-tagged has a calculated MW of 24 kDa. In SDS-PAGE migrates as 24-27 kDa, reducing conditions.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Prss3 protease, serine, 3 [ Mus musculus ]
• Official Symbol: Prss3
• Synonyms: PRSS3; protease, serine, 3; mesotrypsin; trypsin 3; trypsinogen 15; Tb; MTG; TRY4; Try3; MGC176265
• Gene ID: 22073
• mRNA Refseq: NM_011645
• Protein Refseq: NP_035775