Active Recombinant Mouse Ror1 Protein (Gln30-Glu403), C-mFc-tagged

• Cat.No.: Ror1-01M
• Product Overview: Mouse ROR1 (Gln30-Glu403) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Description: This gene encodes a receptor tyrosine kinase that has been implicated in nervous system development, specifically in the maintenance of neural progenitor cell fate, neurite extension and synapse formation. The encoded protein, likely a pseudokinase that lacks catalytic activity, may also regulate adipogenesis. Alternative splicing results in multiple transcript variants.
• Source: Mouse myeloma cell line
• Species: Mouse
• Tag: C-mFc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. Recombinant Mouse ROR1 Fc Chimera binds Biotinylated Recombinant Human/Mouse Wnt-5a with and ED50 of 10-60 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 69 kDa
  SDS-PAGE: 88-100 kDa, reducing conditions
• N-terminal Sequence Analysis: No results obtained. Gln30 inferred from enzymatic pyroglutamate treatment revealing Glu31.
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: 12 months from date of receipt, ≤ -20 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Ror1 receptor tyrosine kinase-like orphan receptor 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ror1
• Synonyms: Ror1; receptor tyrosine kinase-like orphan receptor 1; Ntrkr1; 2810404D04Rik; inactive tyrosine-protein kinase transmembrane receptor ROR1; neurotrophic tyrosine kinase, receptor-related 1; tyrosine-protein kinase transmembrane receptor ROR1; EC 2.7.10.1
• Gene ID: 26563
• mRNA Refseq: NM_013845
• Protein Refseq: NP_038873
• UniProt ID: Q9Z139