Active Recombinant Mouse Sdc4 Protein, His-tagged

• Cat.No.: Sdc4-963M
• Product Overview: Recombinant Mouse Sdc4 (Accession # O35988) Glu24-Val146, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: Glu24-Val146
• Predicted N Terminal: Glu24
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.When 5 x 10^4 cells/well are added to mouse Syndecan-4 and human Fibronectin (0.5 µg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 °C. The ED50 for this effect is typically 1 - 4 µg/mL.
• Molecular Mass: Recombinant Mouse Sdc4, His-tagged has a calculated MW of 14.3 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Sdc4 syndecan 4 [ Mus musculus ]
• Official Symbol: Sdc4
• Synonyms: SDC4; syndecan 4; syndecan-4; ryudocan core protein; Synd4; AA959608; AW108331; ryudocan
• Gene ID: 20971
• mRNA Refseq: NM_011521
• Protein Refseq: NP_035651