Active Recombinant Mouse Sema3a Protein, Fc Chimera

• Cat.No.: Sema3a-908M
• Product Overview: Recombinant Mouse Sema3a (Accession # O08665), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Mouse
• Source: CHO
• Tag: Fc
• Predicted N Terminal: Asn21
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones.20-60 ng/mL of Recombinant Mouse Semaphorin 3A Fc Chimera causes >50% growth cone collapse in the presence 10 ng/mL Recombinant Human beta -NGF.
• Molecular Mass: Recombinant Mouse Sema3a, Fc Chimera has a calculated MW of 110.1 kDa (monomer). In SDS-PAGE migrates as 115-120 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Sema3a sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Mus musculus ]
• Official Symbol: Sema3a
• Synonyms: SEMA3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; semaphorin-3A; sema D; sema III; collapsin-1; semaphorin-D; semaphorin III; SemD; SEMA1; Semad; coll-1; Hsema-I
• Gene ID: 20346
• mRNA Refseq: NM_001243072
• Protein Refseq: NP_001230001