Active Recombinant Mouse Tnfsf12 Protein (His110-His249), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Tnfsf12-86M
• Product Overview: Recombinant Mouse Tnfsf12 Protein (His110-His249) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: His110-His249
• Description: TNF-related Weak Inducer of Apoptosis (TWEAK) is a type II membrane protein of the tumor necrosis factor (TNF) family members, high levels expression in mouse peritoneal macrophages. The murine and human TWEAK homologs are very close related (~93% sequence identity in the receptor-binding domain). TWEAK is a 17.3 kDa protein containing 129 residues. TWEAK induce apoptosis through cognate receptor Fn14 activate caspase-8 and caspase-3, in HSC3 cells KATO-III gastric adenocarcinoma cells and IFN-γ-treated HT-29. TWEAK stimulate inflammatory cytokines in HT-29, A375 cells, WI-38 fibroblast sand astrocytes.
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in HUVEC cells. The ED50 for this effect is < 0.2 μg/mL.
• Molecular Mass: The protein has a calculated MW of 18.14 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Tnfsf12 tumor necrosis factor (ligand) superfamily, member 12 [ Mus musculus (house mouse) ]
• Official Symbol: Tnfsf12
• Synonyms: TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis; Dr3l; Apo3l; Dr3lg; Tweak
• Gene ID: 21944
• mRNA Refseq: NM_011614
• Protein Refseq: NP_035744
• UniProt ID: O54907