Active Recombinant Rat TNFSF12 protein, hFc-tagged

• Cat.No.: TNFSF12-0261R
• Product Overview: Recombinant Rat TNFSF12 protein(NP_001001513.2)(Arg105-His249), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: 105-249 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Fc-rat TNFSF12 at 10 μg/ml (100 μl/well) can bind biotinylated human Fc-TNFRSF12A, The EC50 of biotinylated human Fc-TNFRSF12A is 0.12-0.28 μg/ml.
• Molecular Mass: The recombinant rat TNFSF12 comprises 405 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Proteinlength: Arg105-His249
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfsf12 tumor necrosis factor ligand superfamily member 12 [ Rattus norvegicus ]
• Official Symbol: TNFSF12
• Synonyms: TNFSF12; tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis; TWEAK
• Gene ID: 360548
• mRNA Refseq: NM_001001513
• Protein Refseq: NP_001001513