Active Recombinant Mouse Vegfa Protein (Ala27-Lys140), C-His tagged, Animal-free, Carrier-free
Cat.No. : | Vegfa-91M |
Product Overview : | Recombinant Mouse Vegfa Protein (Ala27-Lys140) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free. |
- Specification
- Gene Information
- Related Products
Description : | Vascular Endothelial Growth Factors 121 (VEGF121) is a truncated version of VEGF165, which produced in E. coli is a homodimer, non-glycosylated, polypeptide chain and having a molecular mass of 28.4 kDa. There is three different isoforms (120, 164 and 188 a.a.) found in mouse. VEGF 121 shows that lack basic heparin-binding regions and are freely diffusible. Mouse VEGF121 shares 98% identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively. |
Source : | E. coli |
Species : | Mouse |
Tag : | C-His |
Form : | Lyophilized |
Bio-activity : | Measure by its ability to induce proliferation in HUVEC cells. The ED50 for this effect is < 3 ng/mL. |
Molecular Mass : | The protein has a calculated MW of 15.01 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis). |
Protein Length : | Ala27-Lys140 |
Endotoxin : | < 0.1 EU/μg of the protein by the LAL method. |
Purity : | >95% as determined by SDS-PAGE analysis. |
Storage : | This product is stable after storage at: -20 centigrade for 12 months in lyophilized state from date of receipt. -20 or -80 centigrade for 1 month under sterile conditions after reconstitution. Avoid repeated freeze/thaw cycles. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0. |
Reconstitution : | It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
Shipping : | The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage. |
Gene Name : | Vegfa vascular endothelial growth factor A [ Mus musculus (house mouse) ] |
Official Symbol : | Vegfa |
Synonyms : | VEGFA; vascular endothelial growth factor A; vascular permeability factor; Vpf; Vegf; Vegf120; Vegf164; Vegf188; |
Gene ID : | 22339 |
mRNA Refseq : | NM_001025250 |
Protein Refseq : | NP_001020421 |
UniProt ID : | Q00731 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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