| Description : |
Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. |
| Source : |
P. pastoris |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 of 1-5 ng/mL, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of 2 × 10^5 to 1 × 10^6 units/mg. |
| Molecular Mass : |
38.2kDa, observed by non-reducing SDS-PAGE |
| Protein Length : |
165 |
| AA Sequence : |
APMAEGGGQNHHEVVKFMDVYQRSY CHPIETLVDIFQEYPDEIEYIFKPS CVPLMRCGGCCNDEGLECVPTEESN ITMQIMRIKPHQGQHIGEMSFLQHN KCECRPKKDRARQENPCGPCSERRK HLFVQDPQTCKCSCKNTDSRCKARQ LELNERTCRCDKPRR |
| Endotoxin : |
< 0.5 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by reducing SDS-PAGE. |
| Storage : |
Lyophilized recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) remains stable up to 12 months at -80 centigrade from date of receipt. Upon reconstitution, rhVEGF-A165 should be stable up to 4 week at 4 centigrade or up to 6 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |
