Active Recombinant Rabbit ANGPT2 protein, His-tagged

• Cat.No.: ANGPT2-315R
• Product Overview: Recombinant Rabbit ANGPT2 protein( Lys 275 - Phe 496), fused with His tag, was expressed in HEK293.

Specification

• Species: Rabbit
• Source: HEK293
• Tag: His
• Protein Length: Lys 275 - Phe 496
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 5mM CHAPS, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Rabbit Angiopoietin-2 Protein, His Tag captured on CM5 chip via anti-His antibody can bind Human TIE2, Fc Tag with an affinity constant of 43.9 nM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 27.4 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Oryctolagus cuniculus ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2
• Gene ID: 100353377