Active Recombinant Rabbit IL17 Protein, His-tagged
Cat.No. : | IL17A-440R |
Product Overview : | Recombinant Rabbit IL17A (XP_002714544.1) (Gly24-Ala153) was expressed with a polyhistidine tag at the N-terminus. |
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Description : | IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological pr centigradeesses including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (mon centigradeytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are ass centigradeiated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. |
Source : | Baculovirus-Insect Cells |
Species : | Rabbit |
Tag : | His |
Predicted N Terminal : | His |
Form : | Lyophilized from sterile Lyophilized from sterile 20 mM PBS, 500 mM NaCl, 10 % glycerol, pH 7.0. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized rabbit IL17a-His at 10μg/mL (100μL/well) can bind rat IL17RA-Fc3, the EC50 of rat IL17RA-Fc3 is 1-40 ng/mL. |
Molecular Mass : | The recombinant rabbit IL17A consists 149 amino acids and predicts a molecular mass of 17.3 kDa. |
Protein length : | Gly24-Ala153 |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 85 % as determined by SDS-PAGE. as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Official Symbol : | IL17A |
Synonyms : | IL17A;interleukin 17A;interleukin-17A |
Official Symbol : | IL17A |
Synonyms : | IL17A;interleukin 17A;interleukin-17A |
Products Types
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◆ Lysates | ||
IL17A-001HCL | Recombinant Human IL17A cell lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewGood for receptor studies.
Effective in Th17 differentiation.
High bioactivity in various tissue types.
Q&As (10)
Ask a questionAnimal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.
Inducing IL17A expression in T cells involves culturing them with specific cytokines.
Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.
Neutralizing antibodies block IL17A's effects in cell cultures or animal models.
Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.
IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.
Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.
Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.
Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.
Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.
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