Active Recombinant Rat Btla Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: BTLA-1148RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant rat Btla (NP_998795.1) (Ala6-Tyr183) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Form: Lyophilized
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized rat BTLA-mFc at 10 μg/mL (100 μL/well) can bind mouse HVEM-Fch. The EC50 of mouse EFNA5-Fc is 6.2-14.4 ng/mL.
• Molecular Mass: 43.4 kDa
• Protein length: 384
• N-terminal Sequence Analysis: Lys 30
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Btla B and T lymphocyte associated [ Rattus norvegicus ]
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein
• Gene ID: 407756
• mRNA Refseq: NM_213630
• Protein Refseq: NP_998795