Active Recombinant Rat Csf1r Protein, hFc-tagged

• Cat.No.: Csf1r-535R
• Product Overview: Recombinant Rat Csf1r Protein(D4ACA7) (Met1-Glu510) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized rat CSF1R-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human CSF1-His, The EC50 of biotinylated human CSF1-His is 1.7-3.9 ng/ml.
  Measured by its ability to inhibit mouse CSF induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 50-250 ng/mL in the presence of 2.5 ng/mL Recombinant Mouse M-CSF.
• Molecular Mass: The recombinant rat CSF1R/Fc is a disulfide-linked homodimer. The reduced monomer comprises 732 amino acids and has a predicted molecular mass of 82 kDa. The apparent molecular mass of the protein is approximately 106-116 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: Met1-Glu510
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Csf1r colony stimulating factor 1 receptor [ Rattus norvegicus ]
• Official Symbol: Csf1r
• Synonyms: CSF1R; colony stimulating factor 1 receptor; macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; fms proto-oncogene; proto-oncogene fms; proto-oncogene c-Fms; c-fms; CSF-1R; CSF-1-R; M-CSF-R; MGC125014
• Gene ID: 307403
• mRNA Refseq: NM_001029901
• Protein Refseq: NP_001025072