Active Recombinant Rat Efnb1 protein(Met1-Thr229), hFc-tagged

• Cat.No.: Efnb1-7434R
• Product Overview: Recombinant Rat EFNB1 (P52796) (Met1-Thr229) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Protein length: Met1-Thr229
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized mouse EphB3-His at 10 μg/ml (100 μl/well) can bind RatEFNB1-Fc, The EC50 of RatEFNB1-Fc is 6.1-14.2 ng/ml.
• Molecular Mass: The recombinant Rat EFNB1 comprises 446 amino acids and predicts a molecular mass of 49.4 kDa. The apparent molecular mass of the Rat EFNB1 is approximately 58 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Efnb1 ephrin B1 [ Rattus norvegicus ]
• Official Symbol: Efnb1
• Synonyms: EFNB1; ephrin B1; ephrin-B1; ELK-L; LERK-2; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; LERK2
• Gene ID: 25186
• mRNA Refseq: NM_017089
• Protein Refseq: NP_058785