Active Recombinant Rat IL3 Protein, His-tagged

• Cat.No.: IL3-1093R
• Product Overview: Recombinant rat IL3 (NP_113701.1) (Met 1-Cys 169) was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: Insect Cells
• Tag: His
• Protein Length: 1-169 a.a.
• Predicted N Terminal: Ile 27
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% gly1, 5%~8% trehalose and mannitol.
• Bio-activity: Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 2-8 ng/mL.
• Molecular Mass: The secreted recombinant rat IL3 comprises 153 amino acids with and has a predicted molecular mass of 17.5 kDa. It migrates as an approximately 24 kDa band in reduced SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Il3 interleukin 3 [ Rattus norvegicus ]
• Official Symbol: IL3
• Synonyms: IL3; interleukin 3; interleukin-3; IL-3; MCGF; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor
• Gene ID: 24495
• mRNA Refseq: NM_031513
• Protein Refseq: NP_113701
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserve
• Function: cytokine activity; cytokine activity; cytokine activity; cytokine activity; growth factor activity; growth factor activity; interleukin-3 receptor binding