Species : |
Rat |
Source : |
E.coli |
Tag : |
Non |
Protein Length : |
Phe25-Thr211 |
Form : |
Lyophilized from sterile 40% acetonitrile, 0.1% TFAPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : |
Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells.The ED50 for this effect is typically 1-6 ng/ml. |
Molecular Mass : |
The recombinant rat IL6 consists of 188 amino acids and has a predicted molecular mass of 21.9 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 22 kDa in SDS-PAGE under reducing conditions. |
Purity : |
> 85 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |