Active Recombinant Rat MSLN protein, His-tagged

• Cat.No.: MSLN-0383R
• Product Overview: Recombinant Rat MSLN protein(Asp298-Ser600), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 298-600 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat Mesothelin (298-600), His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 8-125 ng/mL.Immobilized Rat Mesothelin (298-600), His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag with a linear range of 0.5-16 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 35.9 kDa. The protein migrates as 43-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asp298-Ser600
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Msln mesothelin [ Rattus norvegicus ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; ERC/mesothelin; protein expressed in renal carcinoma; pre-pro-megakaryocyte-potentiating factor
• Gene ID: 60333
• mRNA Refseq: NM_031658
• Protein Refseq: NP_113846