Species : |
Rat |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
298-600 a.a. |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Rat Mesothelin (298-600), His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 8-125 ng/mL.Immobilized Rat Mesothelin (298-600), His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag with a linear range of 0.5-16 ng/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 35.9 kDa. The protein migrates as 43-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Asp298-Ser600 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |