Active Recombinant Rat TNF protein, His-tagged

• Cat.No.: TNF-4335R
• Product Overview: Active Recombinant Rat TNF protein(P16599)(80~235 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: 80-235 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: TNFa, being an endogenous pyrogen, is able to inducefever, apoptotic cell death, inflammation and to inhibit tumorigenesis. As reported, TNFacould inhibit the proliferation and induce apoptosis of A549 cells, andtheconcentration of IL-1β and IL-8 in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL, 100ng/mL, 1000ng/mL) for 8h, IL-1β and IL-8 were detected in the cell supernatant by ELISA. Results: After incubation with TNF-a for 8h, IL-1β and IL-8 significantly increasedin the cell supernatant.
• Molecular Mass: 21.0kDa
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Tnf tumor necrosis factor [ Rattus norvegicus ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; Tnfa; RATTNF; TNF-alpha; MGC124630
• Gene ID: 24835
• mRNA Refseq: NM_012675
• Protein Refseq: NP_036807