| Description : |
Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. |
| Source : |
E. coli |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50< 0.05 ng/mL, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 27units/mg. |
| Molecular Mass : |
18.3kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
163 |
| AA Sequence : |
MHHHHHHMLRSSSQNSSDKPVAHVV ANHQVEEQLEWLSQRANALLANGMD LKDNQLVVPADGLYLVYSQVLFKGQ GCPDYVLLTHTVSRFAISYQEKVNL LSAVKSPCPKDTPEGAELKPWYEPI YLGGVFQLEKGDQLSAEVNLPKYLD FAESGQVYFGVIAL |
| Endotoxin : |
< 1 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by reducing SDS-PAGE. |
| Storage : |
Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-slpha) remains stable up to 12 months at -80 centigrade from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 4 week at 4 centigrade or up to 6 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |
