Active Recombinant Rat TNFSF4 protein, His-tagged

• Cat.No.: TNFSF4-6204R
• Product Overview: Recombinant Rat TNFSF4 protein(Gln 49 - Leu 199), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Gln 49 - Leu 199
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Rat OX40, Fc Tag at 2 μg/mL (100 μL/well) can bind Rat OX40 Ligand, His Tag with a linear range of 20-78 ng/mL. Immobilized Rat OX40 Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Rat OX40, Fc Tag with a linear range of 0.2-5 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 53.8 kDa. The protein migrates as 58-68 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfsf4 tumor necrosis factor (ligand) superfamily, member 4 [ Rattus norvegicus ]
• Official Symbol: TNFSF4
• Synonyms: TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; OX40 ligand; Ox40l
• Gene ID: 89814
• mRNA Refseq: NM_053552
• Protein Refseq: NP_446004