Active Recombinant Rhesus IFNA2 protein, His-tagged
Cat.No. : | IFNA2-231R |
Product Overview : | Recombinant Rhesus IFNA2 protein(NP_001129266.1) (Met1-Glu188) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus. |
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Species : | Rhesus macaque |
Source : | HEK293 |
Tag : | His |
ProteinLength : | Met1-Glu188 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured in antiviral assay using WISH human amnion cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.4-4 pg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Rhesus IFNA2-His at 10 μg/ml (100 μl/well) can bind human IFNAR2-Fc with a linear range of 0.31-2.5 μg/ml. |
Molecular Mass : | The recombinant rhesus IFNA2 comprises 176 amino acids and has a calculated molecular mass of 21 KDa. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C. |
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Not For Human Consumption!
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