| Species : |
S. tuberosum |
| Source : |
E.coli |
| Description : |
Apyrase is a highly active ATP-diphosphohydrolase that catalyzes the sequential hydrolysis of ATP to ADP and ADP to AMP releasing inorganic phosphate. |
| Bio-activity : |
3,000 units/mg |
| Molecular Mass : |
47 kDa |
| Applications : |
Highly efficient degradation of ATP to AMP.
Removal of deoxynucleotides in DNA pyrosequencing between cycles.
Conversion of 5´ triphosphorylated RNA to ligatable monophosphorylated form that can be used for 5´ RNA adaptor ligation.
Conversion of 5´ triphosphorylated RNA to 5´ exonuclease XRN-1bsensitive monophosphorylated RNA. |
| Notes : |
1. Apyrase has a higher ratio of activity for ATP:ADP (14:1).
2. Apyrase is a calcium-activated enzyme. It is approximately 50% active when Mg2+ substitutes Ca2+ in Apyrase Reaction Buffer.
3. As a metal-dependent enzyme, Apyrase can be inhibited by EGTA and EDTA.
4. The activity of Apyrase is approximately 30% in NEBuffers r1.1, r2.1, r3.1 and rCutSmart Buffer.
5. Apyrase does not remove 5'caps from eukaryotic mRNA. |
| Unit Definition : |
One unit is defined as the amount of enzyme that catalyses the release of 1 μmol of inorganic phosphate from ATP (1 mM) in 1X Apyrase Reaction Buffer in 1 minute at 30 centigrade in a total reaction of 50 μL. |
| Reaction Buffer : |
1× Apyrase Reaction Buffer, Incubate at 30 centigrade.
1× Apyrase Reaction Buffer: 20 mM MES, 50 mM NaCl, 5 mM CaCl2, 1 mM DTT, 0.05% Tween 20, (pH 6.5 at 25 centigrade) |
| Storage Buffer : |
20 mM MES, 50 mM NaCl, 0.1 mM CaCl2, 1 mM DTT, 0.1% Tween 20, 50% Glycerol, pH 6.5 at 25 centigrade. |
| Heat Inactivation : |
65 centigrade for 20 minutes |
| Feature : |
Enzyme of highest purity, efficiency and quality
Tested in updated range of more stringent quality controls
Supplied at 10-fold higher concentration in the optimized storage buffer
Supplied with new optimal reaction buffer
Provided with updated, detailed characterization
Discovered and characterized a new function: the ability to convert 5' triphosphorylated RNA to monophosphorylated form, which can be ligated or removed by 5' exonuclease |
