Active Recombinant Yeast XRN1 Protein

• Cat.No.: XRN1-01Y
• Product Overview: Purified from E. coli carrying a plasmid overexpressing the yeast XRN-1 gene

Specification

• Species: Yeast
• Source: E.coli
• Tag: Non
• Description: XRN-1 is highly processive 5´→3´ exoribonuclease, requiring 5´ monophosphate. It also acts on 5´ monophosphate ssDNA with greatly reduced efficiency.
• Reaction Condition: 1× Buffer 3, Incubate at 37 centigrade. 1× Buffer 3: 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT, (pH 7.9 at 25 centigrade)
• Unit Definition: One unit is defined as the amount of enzyme required to digest 1 μg of phosphorylated yeast RNA in one hour at 37 centigrade.
• Application: Tested for the absence of DNases and RNases
• Note: It is recommended to add mouse RNase inhibitor to a final concentration of 1 unit/μL in the reaction to prevent nonspecific RNA degradation.
• Storage Buffer: Supplied with 10X Reaction Buffer (20 mM Tris-HCl, 500 mM NaCl, 2 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% (w/v) Triton X-100)
• Reference: 1. Stevens, A (1980). Biol. Chem. 255, 3080-3085.

Gene Information

• Gene Name: XRN1 chromatin-binding exonuclease XRN1 [ Saccharomyces cerevisiae S288C ]
• Official Symbol: XRN1
• Synonyms: XRN1; chromatin-binding exonuclease XRN1; DST2; KEM1; RAR5; SEP1; SKI1; chromatin-binding exonuclease XRN1; Evolutionarily-conserved 5'-3' exonuclease; deNADing enzyme that modulates mitochondrial NAD-capped RNA; component of cytoplasmic P-bodies involved in mRNA decay; positively regulates transcription initiation and elongation; involved in microtubule-mediated processes, filamentous growth, rRNA maturation, telomere maintenance, tRNA intron turnover; negative regulator of autophagy; activated by glycolytic flux and scavenger decapping enzyme Dcs1p; role in preventing L-A mycovirus pathogenesis
• Gene ID: 852702
• mRNA Refseq: NM_001181038
• Protein Refseq: NP_011342
• UniProt ID: P22147