Active Streptavidin-coupled magnetic Beads

• Cat.No.: Streptavidin-436M

Specification

• Appearance: lyophilized powder mixture
• Particle size: 2.8µm
• Bio-activity: Immobilized 0.04 μg /Test of Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free to the Streptavidin-Magnetic Beads (used for MPCLIA) (10 μg beads/Test), incubated with 100 μL /Test of Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade at increasing concentration coupled to Anti-Mouse IgG-Acridine ester (0.04 μg /Test). Detection was performed with sensitivity of 0.06 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S).
• Coupled amount of protein: About 600 pmol Streptavidin / mg Beads
• Capacity: 5-20 μg biotinylated proteins or molecules / mg Beads
• Background: The Streptavidin-Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin (SA). The beads can be used to capture the biotinylated proteins or other molecules, because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidin, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI). The Streptavidin-Magnetic Beads is easy to capture the biotinylated proteins or other molecules in Chemiluminescence procedures, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
• Application: The Streptavidin-Magnetic Beads is used to capture the biotinylated proteins or other molecules, it can combination with Acridinium ester markers but not Streptavidin Acridinium ester in chemiluminescence technology, The Acridinium ester markers such as Anti-Mouse IgG or Anti-human IgG-Acridinium ester can capture the antibodies, this allows detection of antigen and antibody binding or antibody screening.
• Storage: Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.

Reviews

12/09/2022

Its versatility in integrating with other laboratory reagents streamlined our workflow and improved efficiency.

08/07/2020

The product's customizability allowed us to fine-tune experiments to match our specific research objectives.

03/13/2020

Tailoring the product to our unique experimental conditions enhanced the precision of our results.

Q&As

How does Streptavidin differ from natural streptavidin in its formulation?

Streptavidin formulations often include shortened variants (core streptavidin proteins) with shortened N and C termini compared to natural streptavidin. These variants offer improved characteristics in terms of homogeneity, solubility, resistance to protein hydrolysis, and accessibility of the biotin-binding pocket.

What is Streptavidin?

Streptavidin is a 55kDa tetrameric protein secreted by Streptomyces avidinii, known for its strong binding affinity with biotin.

What is the binding partner of Streptavidin?

Streptavidin tightly binds to biotin (sc-204706).

What is the dissociation constant (Kd) of the Biotin-Streptavidin complex?

The dissociation constant (Kd) for the Biotin-Streptavidin complex is approximately ~10^15M, indicating an exceptionally strong binding affinity.

What are the key features of the Streptavidin/Biotin system?

The Streptavidin/Biotin system exhibits one of the largest observed binding free energies, making it suitable for non-covalent binding of proteins and small ligands in aqueous solutions. The complex is also highly stable over a broad range of temperature and pH values.

What is the origin of Streptavidin?

Streptavidin is produced by Streptomyces avidinii.

Why is Streptavidin widely used in molecular biology?

Streptavidin is widely used in molecular biology due to its unique high affinity for biotin, making it a valuable tool for various applications.