Adenosine Deaminase Fluorometric Activity Assay Kit

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Adenosine Deaminase Fluorometric Activity Assay Kit

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Cat.No. : Kit-2148
Product Overview : ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 µU recombinant Adenosine Deaminase. The range of the assay is from 10-100 µU.
Size : 100 assays
Applications : Detection of Adenosine Deaminase activity
Storage : Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.ADA Assay Buffer (10x): Make 1x Assay Buffer by adding one part 10x Assay Buffer to nine parts deionized water. Store at -20°C or 4°C. Bring to 37°C before use.ADA Convertor and ADA Developer: Reconstitute each with 210 µl ADA Assay Buffer and mix gently by pipetting. Briefly centrifuge to collect the contents at the bottom of the tube. Aliquot and store at -20°C. Avoid repeated freeze/thaw.ADA Substrate: Aliquot and store at -20°C. Avoid repeated freeze/thaw.ADA Positive Control: Reconstitute with 22 µl ADA Assay Buffer and mix gently by pipetting. Briefly centrifuge to collect the contents at the bottom of the tube. Aliquot and store at -20°C. Avoid repeated freeze/thaw.

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